Laboratory of Applied Microbiology Biological Faculty of the Sofia University "St. Kliment Ohridski" (2)

The Laboratory of Applied Microbiology, belonging to Department of General and Industrial Microbiology is specialized in studies on fungi biology comprising the following investigation approaches:

  1. Isolation and identification of fungi: collection of more than 250 strains (yeasts and fungi) isolated and taxonomically characterized by classical and molecular methods from different biotopes in Bulgaria, Russia, Vietnam, China, Taiwan;
  2. Physiological and biochemical investigation using different methods of cultivation (batch, fed-batch, continuous), kinetic studies, optimization and scale up of the fermentation processes in respect to specific bioproducts synthesis;
  3. Down-stream processing of fungal biomass through: separation, disintegration; membrane filtration (micro-, ultrafiltration, reverse osmosis); chromatography (analytical, batch) analyses; electrophoretical studies, freeze- and spray-drying;
  4. Utilization of nonconventional substrates (whey, wastes from distillery, corn steep liquor, methanol, ethanol, glycerol, phenol, toluene, n-alkanes) and their biotechnological recycling through application as a feedstock in fungal fermentations for production of biologically active substances;
  5. Study the biosynthesis and production of exo/endo-cellular enzymes (cellulases, xyllanases, amylases, proteases, a/b galactosidases, superoxide dismutase, catalase, D-amino acid oxidase, Glutathione-S-transferase, etc.), lipids, polyketides’ derivatives (pigments, monacolins and dihiromonacolins), bioflavors;
  6. Investigation of thermotolerance/thermphylness phenomena; resistance to fungal antibiotics; resistance to high concentrations of metal ions (Cu, Zn, Ni, Co, Cd) and ability for intracellular accumulation of Cd nanoparticles;
  7. Exploration the effect of industrial and ecological stress factors on the efficiency of fermentation processes and the quality of the final products: study of the molecular mechanisms, providing robustness and metabolic flexibility of industrially important yeast microorganisms applying classical and modern microbiological, biochemical, molecular biological and bioinformatics approaches;
  8. Monitoring of stress-related genes during yeast cultivation process under exposure to oxidative damage;
  9. Genetic and Molecular Biology analyses: chromosomal and extrachromosomal DNA manipulations for study of strains’ biodiversity, metabolic capabilities and performance of genetic control of industrial yeast fermentations;
  10. Bioinformatics studies: application of improved version of the programme "Tandem Analyser" for mapping of tandemly repeated genes in the genomes of Ascomycetous yeasts for phylogenetic purposes, classification according to protein families and intergenic spaces.